The OLYMPUS FV1000 is designed for high resolution, confocal observation of both fixed and living cells it is equipped with an Incubation Chamber and regulated CO2 supply for live cell work.
The BX43 supports the folowing observation methods brightfield, and fluorescence with attached DP 74 Digital Camera
BSI_Olympus BX43 Upright Microscope Room G.12 BSI
BSI_Olympus BX53 Upright Research Microscope G12
Capacity: 1
Features: - Flatscreen Monitor
Olympus BX53 upright research microscope is fully equiped for high resolution Brightfield, DIC, Darkfield and Fluorescence specimen imaging. The objectives are fully corrected for maximum resolution and brightness of specimens mounted on slides and coverslipped. The attached DP 74 camera system includes, spot metering, automatic exposure control, and zoom magnification
BSI_Olympus BX53 Upright Research Microscope G12
WGB_OLYMPUS IX 71 INVERTED WGB 4.001C.
Capacity: 0
Features:
Located in WGB room 4.001C.
WGB_OLYMPUS IX 71 INVERTED WGB 4.001C.
BSI_OLYMPUS IX73 Inverted Microscope G14
Capacity: 1
Features:
Inverted System with Attached DP72Digital Camera system. Ideal for imaging live cells in culture. Brightfield, Phase Contrast and Fluorescence
BSI_OLYMPUS IX73 Inverted Microscope G14
BSI_Tissue Embedding Console System TissueTek BSI G.16
Capacity: 0
Features:
BSI_Tissue Embedding Console System TissueTek BSI G.16
BSI_Leica RM2135 Rotary Microtome BSI_G 16
Capacity: 0
Features: - Flatscreen Monitor
Leica RM2135 Rotary Microtome G.16
It depends really on the size of the cartridge you want to use, small samples will fit a roughly measured 5mm dept, 27mm wide, 40mm long cartridge. Larger cartridges have a 10mm depth, with the other dimensions remaining the same. Usually 10-15 large cartridges can fit into a basket.
For paraffin embedding samples should be collected and stored in 4% Paraformaldhyde short term. For long term storage in 70% ethanol is recommended.
Parrafin embedding processing in the BSI, EM prep lab G.16
1. Dehydration and permeation in molten wax in the Histokinett http://www.leicabiosystems.com/specimen-preparation/tissue-processing/details/product/leica-tp1020/
(All automated, usually processed 10-15 samples on an overnight cycle 21hrs duration)
2. Wax blocking of samples on Tissue tec http://www.sakura-americas.com/products/tisstek-tec5.html
(Manual, processing is not difficult, need 2hrs max to process 10-15 samples)
3. Sectioning of samples on rotary microtome http://www.gmi-inc.com/leica-rm-2135-biocut-rotary-microtome.htm
(Manual, processing can be difficult especially in warmer weather, need a few days to section 10-15 blocks, depending on your method of sectioning)
BSI_Leica RM2135 Rotary Microtome BSI_G 16
BSI_Jeol Scanning Electron Microscope JSM-5510 BSI G.08
Capacity: 1
Features:
The Jeol Scanning Electron Microscope (JSM 5510) operates at voltages up to 30kV, and has an Oxford Instruments Energy Dispersive XRay Spectroscopy detector attachment for elemental analysis.
BSI_Jeol Scanning Electron Microscope JSM-5510 BSI G.08
BSI_Jeol 2000FXII Transmission Electron Microscope (TEM) BSI G.08 OUT OF ORDER
Capacity: 0
Features:
The Jeol 2000FXII operates at voltages up to 200kV. This is a high resolution instrument for TEM imaging, Scanning Transmission Electron Microscopy (STEM) and electron diffraction.
BSI_Jeol 2000FXII Transmission Electron Microscope (TEM) BSI G.08 OUT OF ORDER
WGB_ANATOMICAL IMAGING SYSTEM CANON RP_TETHERED PC_LIGHTING AND CAMERA SUSPENDED STAND
Capacity: 0
Features:
Canon EOS RP Body + RF 24-105mm IS STM Lens. PC with EOS and Canon Photo Professional software. Tethering cable Lights and Customised camera
support
WGB_ANATOMICAL IMAGING SYSTEM CANON RP_TETHERED PC_LIGHTING AND CAMERA SUSPENDED STAND
BSI_Cryostat 1 BSI G.16
Capacity: 0
Features:
Leica Cryostat 1
The cryostat is designed primarily for the demanding needs of cryosectioning systems in the field of biomedical, neuro-anatomical and pharmaceutical research. Particularly when working with delicate specimens - for example brain samples in neuroscience.
BSI_Cryostat 1 BSI G.16
BSI_IMARIS Workstation BSI 1.36
Capacity: 1
Features:
Imaris provides functionality for data visualization, segmentation, analysis and interpretation of 3D and 4D microscopy datasets, three and four dimensional multichannel images of any size.
Combining large sample overview and working distance with high resolution, the Leica M80 stereo microscope offers 8:1 zoom for detailed imaging of a variety of samples. Ergonomic accessories and modular options, including near vertical illumination, provide optimal user comfort and flexibility for your workspace
